[보고서자료(資料)]Plasmid DNA isolation and & Gel electrophoresis 보고서
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작성일 19-07-10 10:25
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[레포트자료]Plasmid DNA isolation and & Gel electrophoresis 보고서 , [레포트자료]Plasmid DNA isolation and & Gel electrophoresis 보고서기타레포트 , 레포트자료 Plasmid DNA isolation and & Gel electrophoresis
Plasmid DNA isolation and & Gel electrophoresis
Submitted by: choi you jung
Section number: 005
Date of class : 5/3
Teacher: Park eun ok
Introduction
plasmids are often used to purify a specific sequence, since they can easily be purified away from the rest of the genome. For their use as vectors, and for molecular cloning, plasmids often need to be isolated.
There are several methods to isolate plasmid DNA from bacteria, the archetypes of which are the miniprep and the maxiprep/bulkprep.[2] The former can be used to quickly find out whether the plasmid is correct in any of several bacterial clones. The yield is a small amount of impure plasmid DNA, which is sufficient for analysis by restriction digest and for some cloning techniques.
In the latter, much larger volumes of bacterial suspension are grown from which a maxi-prep can be performed. Essentially this is a scaled-up miniprep followed by additional purification. This results in relatively large amounts (se…(투비컨티뉴드 )
[보고서자료(資料)]Plasmid DNA isolation and & Gel electrophoresis 보고서
레포트자료,Plasmid,DNA,isolation,and,&,Gel,electrophoresis,기타,레포트
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[보고서자료(資料)]Plasmid DNA isolation and & Gel electrophoresis 보고서
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